show Abstracthide AbstractCHM1 and CHM13 are haploid human cell lines. This study sequenced the two cell lines on HiSeq-X10 with the standard protocol at Broad Institute, and sequenced a pseudo-diploid sample by experimentally mixing CHM1 and CHM13 DNA at approximately 50-50%. Incorporating public CHM1 and CHM13, including whole-genome PacBio assemblies for each cell line, this study aims to construct a comprehensive truth data set and data resource for evaluating and improving small and structural variant calling accuracy. Note: please contact the submitters if you want to use this data set in a publication.